TCR gamma/delta Antibody (APC)

SKU: orb1952611

Description

TCR gamma/delta Antibody (APC)

Images & Validation

• Tested Applications: FC
• Reactivity: Human, Primate
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. The reactivity of clone B1 (anti-TCR gamma/delta) may be affected by co-staining with an anti-human CD3 mAb, which is likely to block the epitope recognized by clone B1. When using antibody B1 in a panel that includes CD3, it is recommended to apply a staining protocol in which the B1 mAb is added to the sample and incubated for approximately 15 minutes before adding the remaining antibodies. No washing is required between the staining steps.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: B1
• Target: TCR gamma/delta
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TCRG/D

Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells stained using anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human TCR gamma/delta (B1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD3 positive TCR gamma/delta positive T cells (red-filled) from CD3 negative TCR gamma/delta negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR gamma/delta (B1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (B1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Quick Database Links

Gene Symbol

TCR gamma/delta

NCBI Gene ID

35