IL-2 Antibody (PE)

• Catalog Number: orb1742780
• Category: Antibodies
• Description: IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However, IL-2 also stimulates growth and differentiation of B cells, NK cells, monocytes and other cell types, such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein.
• Target: IL-2
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Conjugation: PE
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: recombinant human IL-2
• UniProt ID: P60568
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
• Clone Number: 35C3
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: Interleukin 2
• Note: For research use only
• Entrez: 3558
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood) and intracellular staining using anti-human IL-2 (35C3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human IL-2 positive CD3 positive lymphocytes (red-filled) from IL-2 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).