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Catalog Number | orb44861 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to Human IgM. |
Clonality | Monoclonal |
Clone Number | CH2 |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Purified human IgM. |
Antibody Type | Primary Antibody |
Concentration | 0.2 mg/ml |
Purity | Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography. |
Conjugation | HRP |
Target | IgM |
RRID | AB_10995875 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal |
Alternative names | Anti-IgM antibody Read more... |
Note | For research use only |
Application notes | Western blotting: Recommended dilution: 1 μg/ml. Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining. |
Expiration Date | 12 months from date of receipt. |
Western blotting detection (reducing conditions) of IgM in human plasma using anti-human IgM (CH2) peroxidase conjugate.
ELISA analysis of IgM in human serum using capture antibody MA2 and HRP-conjugated detection antibody CH2. Three different dilutions of human serum were used, and 10% BSA as negative control.