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IgM Antibody (FITC)

SKU: orb44862

Description

Mouse Monoclonal to Human IgM.

Research Area

Stem Cells

Images & Validation

Tested ApplicationsFC
ReactivityHuman
Application Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml. Extracellular and intracellular staining.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1 kappa
Clone No.CH2
ImmunogenPurified human IgM.
TargetIgM
PurificationPurified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ConjugationFITC

Storage & Handling

StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

immunoglobulin M

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IgM Antibody (FITC)

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 µg/ml) and anti-human CD19 (LT19) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

IgM Antibody (FITC)

Flow cytometry multicolor staining pattern of human lymphocytes using anti-human CD19 (LT19) PE-Cy™7 antibody (surface staining, 4 µl reagent / 100 µl of peripheral whole blood) anti-human IgM (CH2) FITC antibody (intracellular staining, concentration in sample 3 µg/ml) after blocking of surface IgM using anti-human IgM (CH2) APC antibody (surface staining, concentration in sample 10 µg/ml).

IgM Antibody (FITC)

Separation of human IgM positive CD19 positive B-cells (red-filled) from IgM negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 µg/ml).

IgM Antibody (FITC)

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 µg/ml).

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

FC
Flow Cytometry
View Protocol

IgM Antibody (FITC) (orb44862)

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0.1 mg
$ 310.00
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