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Anti-Hu IgM FITC
Catalog Number: orb44862
| Catalog Number | orb44862 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to Human IgM. |
| Clonality | Monoclonal |
| Clone Number | CH2 |
| Tested applications | FC |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | Purified human IgM. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | FITC |
| Target | IgM |
| RRID | AB_10987763 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-IgM antibody Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 1-5 μg/ml. Extracellular and intracellular staining. |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 µg/ml) and anti-human CD19 (LT19) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).
Flow cytometry multicolor staining pattern of human lymphocytes using anti-human CD19 (LT19) PE-Cy™7 antibody (surface staining, 4 µl reagent / 100 µl of peripheral whole blood) anti-human IgM (CH2) FITC antibody (intracellular staining, concentration in sample 3 µg/ml) after blocking of surface IgM using anti-human IgM (CH2) APC antibody (surface staining, concentration in sample 10 µg/ml).
Separation of human IgM positive CD19 positive B-cells (red-filled) from IgM negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 µg/ml).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 µg/ml).
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