IgG (Fc) Antibody

SKU: orb44867

Description

Mouse Monoclonal to Human IgG (Fc).

Images & Validation

• Tested Applications: ELISA, FC, ICC, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: EM-07
• Immunogen: Fusion protein of human IgG Fc fragment.
• Target: IgG (Fc)
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Immunoglobulin G Fc fragment

Western blotting detection of IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum, with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B).

Flow cytometry multicolor surface staining of human peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).

Separation of human monocytes (red-filled) from CD3 negative IgG (Fc) positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 µg/ml, GAM APC). The pattern varies depending on the blood donor.

Flow cytometry surface staining pattern of human peripheral whole blood using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 µg/ml, GAM APC). The pattern varies depending on the blood donor.

Flow cytometry surface staining pattern of human peripheral whole blood using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 µg/ml, GAM APC). The pattern varies depending on the blood donor.

Quick Database Links

Gene Symbol

IgG (Fc)

NCBI Gene ID

35