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Catalog Number | orb44857 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to Human IgE. |
Clonality | Monoclonal |
Clone Number | BE5 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Purified human IgE. |
Antibody Type | Primary Antibody |
Concentration | 0.1 mg/ml |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | PE |
Target | IgE |
RRID | AB_10996079 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-IgE antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 2-5 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgE (BE5) PE antibody (concentration in sample 1.67 µg/ml).
Flow cytometry multicolor surface staining pattern of human peripheral whole blood stained using anti-human CD45 (MEM-28) Pacific Blue™ antibody (4 µl reagent / 100 µl of peripheral whole blood) and anti-human IgE (BE5) PE antibody (concentration in sample 1.67 µg/ml).
Separation of human basophils (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgE (BE5) PE antibody (concentration in sample 1.67 µg/ml).