Granzyme A Antibody

• Catalog Number: orb1088500
• Category: Antibodies
• Description: Granzyme A is a serine protease expressed in the cytoplasmic granules of T cells and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Similarly to granzyme B, granzyme A acts to destroy the target cells by proteolysis of their particular components. In case of granzyme A the targets are e.g. APEX1 (it destroys its oxidative repair activity), and nucleosome assembly protein SET (it disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA).
• Target: Granzyme A
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Full length human granzyme A
• UniProt ID: P12544
• Tested applications: FC, ICC, IP
• Application notes: Flow cytometry: Recommended dilution: 5-10 μg/ml, intracellular staining.
• Antibody Type: Primary Antibody
• Clone Number: CB9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HFSP, CTLA3, GrA
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 3001

Separation of human Granzyme A positive NK cells (red-filled) from Granzyme A negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Granzyme A (CB9) purified antibody (concentration in sample 5.0 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme A (CB9) purified antibody (concentration in sample 5.0 μg/ml, GAM APC).