Galectin-9 Antibody (PE)

• Catalog Number: orb1993741
• Category: Antibodies
• Description: Galectin-9 is a glycan-binding protein, which is expressed in three main isoforms of 49 aa, 27 aa, and 15 aa. It can be detected on the cell surface, as well as intracellularly, or in a secreted form. On the cell surface, galectin-9 plays roles in contacts with other cells and with extracellular matrix. It is expressed on multiple cell types, but mainly on Treg cells, activated Th cells and some cancers. Its secreted form acts like a cytokine with immunomodulatory and immunosuppresive functions. Massive and inadequate production of galectin-9, associated with some viral infections or cancers, can counteract immune reactions to these illnesses. High levels of galectin-9 expression lead to poor prognosis of cancer patients.
• Target: Galectin-9
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: PE
• Reactivity: Human, Primate
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: recombinant M-type splicing variant of human galectin 9
• UniProt ID: O00182
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
• Clone Number: 9M1-3
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: HUAT, LGalS9A, LEG9
• Note: For research use only
• Entrez: 3965
• Expiration Date: 12 months from date of receipt.

Separation of Jurkat cells stained using anti-human Galectin-9 (9M1-3) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension, red-filled) from Jurkat cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 µg/ml, same as Galectin-9 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining).