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Item 1 of 3
Anti-Hu CX3CR1 FITC
Catalog Number: orb2704157
| Catalog Number | orb2704157 |
|---|---|
| Category | Antibodies |
| Description | CX3CR1 (fractalkine receptor) serves as a regulator of leukocyte trafficking. Its ligand CX3CL1 (fractalkine) can serve bots as chemoatractant (soluble) and as adhesive molecule (membrane bound). Binding to the cell surface expressed ligand leads to rapid induction of firm adhesion of CX3CR1-positive cells without requiring selectin-mediated rolling or activation of integrins. This interaction is important for trafficking of cytotoxic effector lymphocytes regardless of their lineage and mode of target cell recognition, through inflamed endothelium. Similarly, it mediates monocyte/macrophage recruitment to inflamed atherosclerotic plaques, as well as in their homing to non-inflamed tissues, where they can differentiate into dendritic cells. CX3CR1 also promotes cell survival, plays a key role in brain microglia, synapse maturation and regulation of inflammatory response in the central nervous system, it acts as a negative regulator of angiogenesis, and serves as an important regulator of the gut microbiota by controlling immunity to intestinal bacteria and fungi. |
| Target | CX3CR1 |
| Clonality | Monoclonal |
| Isotype | Rat IgG2b kappa |
| Conjugation | FITC |
| Reactivity | Human, Primate |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Immunogen | human CX3CR1 transfectants |
| UniProt ID | P49238 |
| Tested applications | FC |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Clone Number | 2A9-1 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative names | V28, CCRL1, GPR13, CMKDR1, GPRV28, CMKBRL1, fracta Read more... |
| Note | For research use only |
| Entrez | 1524 |
| Expiration Date | 12 months from date of receipt. |
Separation of human CXCR1 positive CD3 negative lymphocytes (red-filled) from CXCR1 negative CD3 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CX3CR1 (2A9-1) FITC antibody (concentration in sample 0.56 µg/ml).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CX3CR1 (2A9-1) FITC antibody (concentration in sample 0.56 µg/ml).
Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human CX3CR1 (2A9-1) FITC antibody (concentration in sample 0.56 µg/ml).
