CD99 Antibody (PE)

SKU: orb154511

Description

Mouse monoclonal antibody conjugated to PE which recognizes CD99 that belongs to the CD99 family. It plays role in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. CD99 is expressed in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation.

Research Area

Epigenetics

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a kappa
• Clone No.: 3B2/TA8
• Immunogen: Human thymocytes
• Target: CD99
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MIC2, HBA71, blood group Xg

Separation of human CD99 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD99 (3B2/TA8) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD99 (3B2/TA8) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Quick Database Links

Gene Symbol

CD99

UniProt

P14209

NCBI Gene ID

4267