Anti-Hu CD95 Purified

• Catalog Number: orb43957
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD95
• Clonality: Monoclonal
• Clone Number: LT95
• Tested applications: FC, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: HUT-78 human T cell lymphoma cell line
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD95
• Entrez: 355
• UniProt ID: P25445
• RRID: AB_10995254
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti ALPS 1A antibody, anti ALPS1A antibody, anti
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-12 µg/ml. Excellent. Immunohistochemistry (paraffin sections): Positive tissue: tonsil.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining; Black - standard anti-CD95 monoclonal antibody (DX2); Green - anti-CD95 (LT95). A - JURKAT human peripheral blood T cell leukemia cell line; B - RAMOS human Burkitt lymphoma cell line; C - CEM human leukemia cell line; D - MOLT-4 human acute lymphoblastic T cell leukemia cell line.

Separation of human CD95 positive lymphocytes (red-filled) from CD95 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD95 (LT95) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD95 (LT95) purified antibody (concentration in sample 2 µg/ml) GAM APC.