CD85j Antibody

SKU: orb154415

Description

Mouse monoclonal antibody to CD85j which is a member of the leukocyte immunoglobulin-like receptor (LIR) family, also known as ILT2, LILRB1, and LIR1. CD85j expressed predominantly on myeloid lineage cells. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1.

Images & Validation

• Tested Applications: FC, IP, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Clone No.: GHI/75
• Immunogen: Hairy cell leukaemia cells
• Target: CD85j
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

ILT2, LIR1, MIR7, PIRB, ILT-2

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD85j (GHI/75) purified antibody (concentration in sample 1 μg/ml) GAM APC and anti-human CD19 (LT19) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD85j positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD85j(GHI/75) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD85j(GHI/75) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD85j

UniProt

Q8NHL6

NCBI Gene ID

10859