Anti-Hu CD74 PE

• Catalog Number: orb1570904
• Category: Antibodies
• Description: CD74 (the MHC II-associated invariant chain, Ii) is a type II transmembrane protein expressed in antigen-presenting cells, that serves as MHC II chaperone which promotes MHC II trafficking from the ER to endocytic compartments, prevents peptide binding in the ER and contributes to peptide editing in the MHC II compartment; it is also an accessory signaling molecule implicated e.g. in malignant B cell proliferation. Stimulation of cell surface CD74 leads to NFkappaB activation, which enables entry of the stimulated cell into the S phase. CD74 binds pro-inflammatory cytokine MIF with high affinity and interacts with CD44. Binding of Vpu, an HIV1 protein, to CD74 modulates MHC II presentation.
• Target: CD74
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: PE
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: SU-DHL-4 lymphoma cells
• UniProt ID: P04233
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Clone Number: LN2
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: p33, DHLAG, HLADG, Ia-gamma
• Note: For research use only
• Entrez: 972

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD19 (4G7) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD74 (LN2) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD74 positive B cells (red-filled) from CD74 negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD74 (LN2) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD74 (LN2) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).