Anti-Hu CD71 Purified

• Catalog Number: orb44381
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD71
• Clonality: Monoclonal
• Clone Number: MEM-75
• Tested applications: FC, ICC, IP
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: NALM-6 human pre-B cell line
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 4 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD71
• Entrez: 7037
• UniProt ID: P02786
• RRID: AB_10990595
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD71 antibody, anti CD71 antigen antibody, an
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human PHA stimulated lymphocytes using anti-human CD3 (UCHT1) PE antibody (20 µl reagent / 100 µl of peripheral whole blood) and anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.56 µg/ml, GAM APC).

Separation of human CD3 positive CD71 positive lymphocytes (red-filled) from CD3 negative CD71 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.56 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.56 µg/ml, GAM APC).

Immunocytochemistry staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).