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Item 1 of 3
Anti-Hu CD63 Biotin
Catalog Number: orb154462
| Catalog Number | orb154462 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody conjugated to Biotin which recognizes is a CD63 that is lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is observed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. CD63 is belongs to the TM4 superfamily of leukocyte glycoproteins. CD63 may participates in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome. |
| Clonality | Monoclonal |
| Clone Number | MEM-259 |
| Tested applications | FC, ICC, IHC-P, IP |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | HPB-ALL T cell line |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Conjugation | Biotin |
| Target | CD63 |
| Entrez | 967 |
| UniProt ID | P08962 |
| Storage | Store at 2-8°C. Do not freeze. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-OMA81H antibody, anti-Granulophysin antibody, Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 1-3 μg/ml; positive material: activated platelets, neutrophils and basophils. |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0.6 μg/ml, Streptavidin APC) and anti-human CD203c (NP4D6) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).
Separation of CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of IgE stimulated human peripheral whole blood using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0.6 μg/ml, Streptavidin APC).
Flow cytometry surface staining pattern of IgE stimulated human peripheral whole blood stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0.6 μg/ml, Streptavidin APC).
