CD59 Antibody

SKU: orb44303

Description

Mouse Monoclonal to CD59.

Research Area

Epigenetics, Stem Cells

Images & Validation

• Tested Applications: FC, IHC-P, IP, WB
• Reactivity: Human, Mouse
• Application Notes:
  Flow cytometry: Recommended dilution: 0.5-4 μg/ml; positive control: blood. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml. Western blotting: Recommended dilution: 1-2 μg/ml; positive control: mouse spleen, non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: MEM-43/5
• Immunogen: Thymocytes and T lymphocytes
• Target: CD59
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

MACIF, MAC-IP, protectin, MIRL, HRF-20, HRF20, 1F5, MIN

Western blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate; Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43; cat. no. orb44294)

Separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).

Immunohistochemistry staining of human skin (paraffin sections) using anti-CD59 (MEM-43/5).

Quick Database Links

Gene Symbol

CD59

UniProt

P13987

NCBI Gene ID

966