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Anti-Hu CD59 Purified
Catalog Number: orb44303
| Catalog Number | orb44303 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to CD59. |
| Clonality | Monoclonal |
| Clone Number | MEM-43/5 |
| Tested applications | FC, IHC-P, IP, WB |
| Reactivity | Human, Mouse |
| Isotype | Mouse IgG2b |
| Immunogen | Thymocytes and T lymphocytes |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Dilution range | Flow cytometry: Recommended dilution: 1 μg/ml; positive control: blood. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml. Western blotting: Recommended dilution: 1-2 μg/ml; positive control: mouse spleen, non-reducing conditions. |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD59 |
| Entrez | 966 |
| UniProt ID | P13987 |
| RRID | AB_10995088 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-CD59 antibody, anti-Protectin antibody Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 1 μg/ml; positive control: blood. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml. Western blotting: Recommended dilution: 1-2 μg/ml; positive control: mouse spleen, non-reducing conditions. |
| Expiration Date | 12 months from date of receipt. |
Western blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate; Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43; cat. no. orb44294)
Separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).
Immunohistochemistry staining of human skin (paraffin sections) using anti-CD59 (MEM-43/5).
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