CD5 Antibody (FITC)

SKU: orb154491

Description

Mouse monoclonal antibody conjugated to FITC which recognizes CD5 that also designated Lyt-1 that has been identified as a transmembrane glycoprotein that is expressed on 70% of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. It plays role as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2. CD5 is expressed at various developmental and activation stages on human B cells.

Research Area

Epigenetics, Stem Cells

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a kappa
• Clone No.: L17F12
• Immunogen: Human acute lymphoblastic leukemia (ALL) T cells
• Target: CD5
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Disclaimer: For research use only

Alternative Names

T1, LEU1

Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD5 (L17F12) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD5 (L17F12) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Quick Database Links

Gene Symbol

CD5

UniProt

P06127

NCBI Gene ID

921