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| Catalog Number | orb154457 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody conjugated to APC which recognizes CD5 that also designated Lyt-1 that has been identified as a transmembrane glycoprotein that is expressed on 70% of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. It plays role as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2. CD5 is expressed at various developmental and activation stages on human B cells. |
| Target | CD5 |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a kappa |
| Conjugation | APC |
| Reactivity | Human |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purification | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Immunogen | Human acute lymphoblastic leukemia (ALL) T cells |
| UniProt ID | P06127 |
| Tested applications | FC |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Antibody Type | Primary Antibody |
| Clone Number | L17F12 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative names | T1, LEU1 |
| Research Area | Epigenetics, Stem Cells |
| Note | For research use only |
| Entrez | 921 |
| Expiration Date | 12 months from date of receipt. |

Flow cytometry analysis (surface staining) of CD5 in human peripheral blood cells with anti-CD5 (L17F12) APC.
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