Anti-Hu CD45 Biotin

• Catalog Number: orb44267
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD45 conjugated to Biotin.
• Target: CD45
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Biotin
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Immunogen: Human thymocytes and T lymphocytes.
• UniProt ID: P08575
• RRID: AB_10994432
• Tested applications: FC, ICC, IHC-P, IP, WB
• Dilution range: Flow cytometry: Recommended dilution: 1 μg/ml.
• Application notes: Flow cytometry: Recommended dilution: 1 μg/ml.Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa).
• Antibody Type: Primary Antibody
• Clone Number: MEM-28
• Storage: Store at 2-8°C. Do not freeze.
• Alternative names: anti CD 45 antibody (Biotin), anti CD45 antibody (
• Note: For research use only
• Entrez: 5788

Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD45 (MEM-28) biotin.

Anti-Hu CD45 Biotin (clone MEM-28) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with biotinylated mouse IgG1 monoclonal antibody MEM-28 (1 µg/ml), followed by IRDye 800CW Streptavidin (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.