Anti-Hu CD43 Purified Low Endotoxin

• Catalog Number: orb154417
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD43 which is the major O-glycosylated cell-surface associated sialoglycoprotein found on the cell membranes of leukocytes. Itbelongs to the surface mucin family which plays a central role in cellular adhesion tumor progression. CD43 is thought to function as a negative regulator of cellular adhesion.
• Clonality: Monoclonal
• Clone Number: MEM-59
• Tested applications: FA, FC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human T lymphocytes.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Functional application: The antibody MEM-59 activates T lymphocytes and also induces apoptosis in hemopoietic progenitor cells. Flow cytometry: Recommended dilution: 1 μg/ml. Immunohistochemistry (paraffin sections): Positive tissue: spleen, thymus, tonsil; recommended dilution: 10 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD43
• Entrez: 6693
• UniProt ID: P16150
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-Leukosialin antibody, anti-Sialophorin antibo
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of human CD43 positive lymphocytes (red-filled) from human CD43 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD43 (MEM-59) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD43 (MEM-59) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD43 (MEM-59).