CD369 Antibody (APC)

• Catalog Number: orb1678978
• Category: Antibodies
• Description: CD369 (dectin-1, beta-glucan receptor) is a 33 kDa type II transmembrane glycoprotein of lectin family, and serves as a part of innate immunity system by binding to beta-glucan polymers, which are typical for yeast and mycobacterial cell walls. CD369 is expressed predominantly on dendritic cells, but it can be detected also on monocytes, macrophages, mast cells, eosinophils, B cells, endothelial cells, and sometimes also on some T cell subsets.
• Target: CD369
• Clonality: Monoclonal
• Isotype: Mouse IgG2a kappa
• Conjugation: APC
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: Extracellular part of human CD369 with hIgG FC tag
• UniProt ID: Q9BXN2
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: 15E2
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: Dectin-1, CLEC7A, BGR, CANDF4, SCARE2
• Note: For research use only
• Entrez: 64581
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD369 (15E2) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD369 (15E2) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).