CD354 Antibody (APC)

• Catalog Number: orb1993788
• Category: Antibodies
• Description: CD354 (TREM-1), a cell surface glycoprotein expressed mainly on monocytes, macrophages, and neutrophils, but also on e.g. endothelial cells or bronchial epithelium, plays an important role in innate immune responses. Upon triggering by its ligands (mainly bacterial and fungal components, but also some viruses, such as Marburg or Ebola virus), it initiates signaling cascades leading to release of pro-inflammatory cytokines and chemokines. CD354 is strongly present in infectious inflammatory lesions (e.g. in folliculitis or impetigo), but not so much in non-infectious inflammatory lesions, (e.g. vasculitis or psoriasis). However, under certain conditions neutrophils can upregulate CD354 also under non-infectious inflammatory conditions, e.g. in rheumatoid arthritis. Soluble CD354 (sTREM-1) is an important biomarker for identification of septic patients, as well as for prediction of their survival.
• Target: CD354
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: APC
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: recombinant human CD354 fused with IgG
• UniProt ID: Q9NP99
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: 6B1
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: TREM-1
• Note: For research use only
• Entrez: 54210
• Expiration Date: 12 months from date of receipt.

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD354 (6B1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD354 (6B1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).