CD34 Antibody

SKU: orb43605

Description

Mouse Monoclonal to CD34.

Research Area

Cardiovascular Disease, Epigenetics, Neuroscience

Images & Validation

• Tested Applications: FC, ICC, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Western blotting: Recommended dilution: 1-2 μg/ml; positive control: Kg-1a, TF-1 cells, non-reducing conditions. Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: placenta endothelium.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 4H11[APG]
• Immunogen: Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
• Target: CD34
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry surface staining pattern of human peripheral whole blood showing CD34 positive stem cells (red) stained using anti-CD34 (4H11[APG]) purified / GAM-APC.

Western bloting analysis of human CD34 using mouse monoclonal antibody 4H11[APG] on lysates of TF-1 cell line and HEK293T/17 cell line (CD34 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD34 monoclonal antibody 4H11[APG] followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for CD34 protein at approximately 110 kDa.

Quick Database Links

Gene Symbol

CD34

UniProt

P28906

NCBI Gene ID

947