Anti-Hu CD34 PerCP

• Catalog Number: orb1993652
• Category: Antibodies
• Description: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
• Target: CD34
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: PerCP
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
• UniProt ID: P28906
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: 4H11[APG]
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Note: For research use only
• Entrez: 947

Separation of human CD34 positive stem cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD34 (4H11[APG]) PerCP antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD34 (4H11[APG]) PerCP antibody (10 µl reagent / 100 µl of peripheral whole blood).