CD34 Antibody (PE)

SKU: orb43608

Description

Mouse Monoclonal to CD34.

Research Area

Cardiovascular Disease, Epigenetics, Neuroscience

Images & Validation

• Tested Applications: FC, ICC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 4H11[APG]
• Immunogen: Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
• Target: CD34
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Separation of human CD34 positive CD45dim stem cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD34 (4H11[APG]) PE antibody (20 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD34 (4H11[APG]) PE antibody (20 µl reagent / 100 µl of peripheral whole blood).

Immunocytochemistry detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 (4H11[APG]) PE.

Quick Database Links

Gene Symbol

CD34

UniProt

P28906

NCBI Gene ID

947