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| Catalog Number | orb1467378 |
|---|---|
| Category | Antibodies |
| Description | CD307a is a type I transmembrane glycoprotein of the Fc receptor family. It contains two ITAM motifs in its cytoplasmic domain. CD307a is expressed mainly on the surface of mature B-cells, and is down-regulated in germinal center B-cells. Expression of CD307a is higher in patients with autoimmune diseases, compared with healthy controls. |
| Target | CD307a |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Conjugation | PE |
| Reactivity | Human |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Immunogen | DNA-immunization followed by a boost with the CD307a transfected cells |
| UniProt ID | Q96LA6 |
| Tested applications | FC |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Antibody Type | Primary Antibody |
| Clone Number | E3 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative names | FcRL1, FcRH1, IRTA5 |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 115350 |

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD307a(E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (4G7) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD307a (E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD307a (E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
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