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Anti-Hu CD30 APC
Catalog Number: orb1952610
| Catalog Number | orb1952610 |
|---|---|
| Category | Antibodies |
| Description | CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response. |
| Clonality | Monoclonal |
| Clone Number | Ber-H8 |
| Tested applications | FC |
| Reactivity | Human |
| Isotype | Mouse IgG1 kappa |
| Conjugation | APC |
| Target | CD30 |
| Entrez | 943 |
| UniProt ID | P28908 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Ki-1, TNFRSF8, CD30L receptor, D1S166E Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Expiration Date | 12 months from date of receipt. |
Separation of K562 cells stained using anti-human CD30 (Ber-H8) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) from K562 cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 5 μg/ml, same as TCR gamma/delta APC concentration, black-dashed) in flow cytometry analysis (surface staining).
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