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| Catalog Number | orb44565 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against CD3 conjugated to PerCP |
| Target | CD3 |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Conjugation | PerCP |
| Reactivity | Human, Primate |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purity | Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Purification | Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Immunogen | human thymocytes followed by Sezary T cells |
| UniProt ID | P07766 |
| RRID | AB_10994056 |
| Tested applications | FC |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining. |
| Antibody Type | Primary Antibody |
| Clone Number | UCHT1 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative names | anti CD 3 antibody (PerCP), anti Cd247 antibody (P Read more... |
| Note | For research use only |
| Entrez | 916 |
Rudolf-Oliveira, Renata Cristina Messores et al. Determination of normal expression patterns of CD86, CD210a, CD261, CD262, CD264, CD358, and CD361 in peripheral blood and bone marrow cells by flow cytometry Immunol Lett, 194, 44-55 (2018)
Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (UCHT1) PerCP antibody (10 µl reagent / 100 µl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (UCHT1) PerCP antibody (10 µl reagent / 100 µl of peripheral whole blood).
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