CD3 Antibody (FITC)

SKU: orb44561

Description

Mouse monoclonal antibody against CD3 conjugated to FITC

Images & Validation

• Tested Applications: FC
• Reactivity: Human, Primate
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Extracellular and intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: UCHT1
• Immunogen: human thymocytes followed by Sezary T cells
• Target: CD3
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Disclaimer: For research use only

Alternative Names

CD3E, T3E, TCRE

Separation of human CD3 positive T cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (UCHT1) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (UCHT1) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).

Quick Database Links

Gene Symbol

CD3

UniProt

P07766

NCBI Gene ID

916