CD29 Antibody

SKU: orb44007

Description

Mouse monoclonal antibody to Integrin beta 1

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: FC, IP, WB
• Reactivity: Canine, Human, Porcine
• Application Notes:
  Western blotting: Recommended dilution: 2 μg/ml; positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; non-reducing conditions, 6% separating gel, 4% stacking gel. Flow cytometry: Recommended dilution: 2 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-101A
• Immunogen: Raji Burkitt's lymphoma cell line
• Target: CD29
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Integrin β1 chain, ITGB1, VLAB, MDF2, FNRB, GPIIA, MSK12

Western blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD29 (MEM-101A). Lane 1: lysate of human PBL; Lane 2: lysate of canine PBL; Lane 3: lysate of porcine PBL.

Separation of human CD29 positive platelets (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD29 (MEM-101A) purified antibody (concentration in sample 3 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD29 (MEM-101A) purified antibody (concentration in sample 3 µg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD29

UniProt

P05556

NCBI Gene ID

3688