CD273 Antibody (PE)

SKU: orb414547

Description

Mouse monoclonal antibody against CD273 conjugated to PE

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a kappa
• Clone No.: 24F.10C12
• Immunogen: human CD273 cDNA
• Target: CD273
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PDCD1LG2, B7DC, Btdc, PDL2, PDCD1L2, bA574F11.2, Butyrophilin, B7-DC,

Flow cytometry multicolor surface staining of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD273 positive CD11c positive cells (red-filled) from CD273 negative CD11c negative cells (black-dashed) in flow cytometry analysis (surface staining) of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension).

Flow cytometry surface staining pattern of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension).

Quick Database Links

Gene Symbol

CD273

UniProt

Q9BQ51

NCBI Gene ID

80380