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Anti-Hu CD273 PE
Catalog Number: orb414547
| Catalog Number | orb414547 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against CD273 conjugated to PE |
| Target | CD273 |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a kappa |
| Conjugation | PE |
| Reactivity | Human |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Immunogen | human CD273 cDNA |
| UniProt ID | Q9BQ51 |
| Tested applications | FC |
| Application notes | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Antibody Type | Primary Antibody |
| Clone Number | 24F.10C12 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative names | Anti-PDCD1LG2 antibody, anti-B7DC antibody, anti-B Read more... |
| Note | For research use only |
| Entrez | 80380 |
Flow cytometry multicolor surface staining of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).
Separation of human CD273 positive CD11c positive cells (red-filled) from CD273 negative CD11c negative cells (black-dashed) in flow cytometry analysis (surface staining) of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension).
Flow cytometry surface staining pattern of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension).
