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Anti-Hu CD273 APC

Catalog Number: orb545692

DispatchUsually dispatched within 5-10 working days
$ 390.00
Catalog Numberorb545692
CategoryAntibodies
DescriptionMouse monoclonal antibody against CD273 conjugated to APC
TargetCD273
ClonalityMonoclonal
IsotypeMouse IgG2a kappa
ConjugationAPC
ReactivityHuman
Buffer/PreservativesStabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
PurityPurified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
PurificationPurified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Immunogenhuman CD273 cDNA
UniProt IDQ9BQ51
Tested applicationsFC
Application notesFlow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Antibody TypePrimary Antibody
Clone Number24F.10C12
StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Alternative namesAnti-PDCD1LG2 antibody, anti-B7DC antibody, anti-B
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NoteFor research use only
Entrez80380
Anti-Hu CD273 APC

Separation of human dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Anti-Hu CD273 APC

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) PE-Cy™7 antibody (4 μl reagent per milion cells in 100 μl of cell suspension).