CD253 Antibody

SKU: orb222728

Description

Mouse monoclonal antibody to CD253

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: FA, FC
• Reactivity: Human
• Application Notes:
  Functional application: The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays. Flow cytometry: Recommended dilution: 1-10 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 2E5
• Immunogen: Recombinant soluble fragment (aa 95-281) of human TRAIL.
• Target: CD253
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TRAIL, Apo-2 ligand, Apo-2L, TNFSF10, APO2L, TNLG6A

Functional applicvation example: Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5). The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL. A - medium B - recombinant TRAIL C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 μg/ml) D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 μg/ml) E - recombinant TRAIL + Isotype mouse IgG1 control

Quick Database Links

Gene Symbol

CD253

UniProt

P50591

NCBI Gene ID

8743