CD235a Antibody (Biotin)

• Catalog Number: orb1088488
• Category: Antibodies
• Description: CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.
• Target: CD235a
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Biotin
• Reactivity: Human, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Immunogen: Membrane preparation from splenic hairy cell leukemia
• UniProt ID: P02724
• Tested applications: FC, IHC-Fr, IHC-P
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: JC159
• Storage: Store at 2-8°C. Do not freeze.
• Alternative names: Glycophorin A, GYPA, GPA, PAS-2, Sialoglycoprotein
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 2993

Separation of human CD235a positive erythrocytes (red-filled) from leukocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD235a (JC159) Biotin antibody (concentration in sample 5 μg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD235a (JC159) Biotin antibody (concentration in sample 5 μg/ml, Streptavidin APC).