Anti-Hu CD206 Purified

• Catalog Number: orb308745
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD206
• Clonality: Monoclonal
• Clone Number: 15-2
• Tested applications: FC, ICC, IHC-Fr, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: Purified human mannose receptor
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD206
• Entrez: 4360
• UniProt ID: P22897
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti MMR antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (concentration in sample 9 μg/ml), GAM PE.

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (concentration in sample 9 μg/ml), GAM PE.

Western blotting analysis of human CD206 using mouse monoclonal antibody 15-2 on lysates of differentiated monocytes (two batches), and on THP-1 and Raji cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD206 was detected around 170 kDa.