CD206 Antibody

SKU: orb308745

Description

Mouse monoclonal antibody to CD206

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: FC, ICC, IHC-Fr, IP, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: 15-2
• Immunogen: Purified human mannose receptor
• Target: CD206
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MMR, MRC1, CLEC13DL

Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (concentration in sample 9 μg/ml), GAM PE.

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (concentration in sample 9 μg/ml), GAM PE.

Western blotting analysis of human CD206 using mouse monoclonal antibody 15-2 on lysates of differentiated monocytes (two batches), and on THP-1 and Raji cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD206 was detected around 170 kDa.

Quick Database Links

Gene Symbol

CD206

UniProt

P22897

NCBI Gene ID

4360