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Anti-Hu CD172ab APC
Catalog Number: orb1786030
| Catalog Number | orb1786030 |
|---|---|
| Category | Antibodies |
| Description | CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells. Extracellular part of CD172b is 90% identical to that of CD172a, but unlike CD172, it has dramatically reduced intracellular domain. |
| Target | CD172ab |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Conjugation | APC |
| Reactivity | Human |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purification | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Immunogen | NIH-3T3 / human CD172a cell line |
| Tested applications | FC |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Clone Number | SE5A5 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative names | PTPNS1, BIT, MFR, SIRPA, SHPS1, SIRPB1, |
| Note | For research use only |
Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD172ab (SE5A5) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD172ab (SE5A5) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
