CD172a Antibody (PE)

SKU: orb699893

Description

Mouse monoclonalantibody against CD172a conjugated to PE

Research Area

Epigenetics

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: 15-414
• Immunogen: Kg-1a cell line
• Target: CD172a
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Disclaimer: For research use only

Alternative Names

PTPNS1, BIT, MFR, SIRPA, SHPS1

Expression profiling on peripheral blood subsets using Anti-human CD172a PE antibody (clone 15-414). HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of anti-human SIGLEC10 PE antibody (clone 15-414, 10 μl reagent / 100 μl of stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Anti-human CD172a PE antibody (clone 15-414) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Mouse monoclonal anti-human CD172a PE antibody (clone 15-414) was used in amount of 10 µl in 100 µl of blood sample (2 x 1^6 cells).

Quick Database Links

Gene Symbol

CD172a

UniProt

P78324

NCBI Gene ID

140885