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Anti-Hu CD172a FITC
Catalog Number: orb1570907
| Catalog Number | orb1570907 |
|---|---|
| Category | Antibodies |
| Description | CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells. |
| Target | CD172a |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Conjugation | FITC |
| Reactivity | Human |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purification | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Immunogen | Kg-1a cell line |
| UniProt ID | P78324 |
| Tested applications | FC |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Clone Number | 15-414 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative names | PTPNS1, BIT, MFR, SIRPA, SHPS1 |
| Note | For research use only |
| Entrez | 140885 |
Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD172a (15-414) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD172a (15-414) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
