CD169 Antibody (APC)

SKU: orb545675

Description

Mouse monoclonal antibody against CD169_Sialoadhesin conjugated to APC

Research Area

Epigenetics

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 7-239
• Immunogen: human rhinovirus 14-infected monocyte-derived dendritic cells
• Target: CD169
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Disclaimer: For research use only

Alternative Names

SIGLEC1, SN, sialoadhesin

Flow cytometry multicolor surface staining of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using anti-human CD169 (7-239) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD169 positive CD11c positive cells (red-filled) from CD169 negative CD11c negative cells (black-dashed) in flow cytometry analysis (surface staining) of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using anti-human CD169 (7-239) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Flow cytometry surface staining pattern of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using anti-human CD169 (7-239) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Quick Database Links

Gene Symbol

CD169

UniProt

Q9BZZ2

NCBI Gene ID

6614