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Catalog Number | orb545675 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against CD169_Sialoadhesin conjugated to APC |
Clonality | Monoclonal |
Clone Number | 7-239 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | human rhinovirus 14-infected monocyte-derived dendritic cells |
Antibody Type | Primary Antibody |
Purity | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | APC |
Target | CD169 |
Entrez | 6614 |
UniProt ID | Q9BZZ2 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-SIGLEC1 antibody, anti-SN antibody, anti-sial Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using anti-human CD169 (7-239) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD169 positive CD11c positive cells (red-filled) from CD169 negative CD11c negative cells (black-dashed) in flow cytometry analysis (surface staining) of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using anti-human CD169 (7-239) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
Flow cytometry surface staining pattern of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using anti-human CD169 (7-239) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).