CD154 Antibody (APC)

SKU: orb1806414

Description

CD154 Antibody (APC)

Images & Validation

• Tested Applications: FC
• Reactivity: Human, Primate
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 24-31
• Immunogen: human CD154 fusion protein
• Target: CD154
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD154 (24-31) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension) and anti-human CD3 (UCHT1) PE antibody (20 μl reagent per million cells in 100 μl of cell suspension).

Separation of human lymphocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (PHA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension).

Flow cytometry surface staining pattern of human stimulated (PHA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension).

Quick Database Links

Gene Symbol

CD154

UniProt

P27548

NCBI Gene ID

21947