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Catalog Number | orb222750 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against CTLA4 conjugated to PE |
Clonality | Monoclonal |
Clone Number | BNI3 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG2a |
Immunogen | Human CD152-IgG heavy chain fusion protein |
Antibody Type | Primary Antibody |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | PE |
Target | CD152 |
Entrez | 1493 |
UniProt ID | P16410 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | anti-ALPS5 antibody, anti-CD 152 antibody, anti-CD Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining of human PHA stimulated lymphocytes stained using anti-human CD152 (BNI3) PE antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).
Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral whole blood stained using anti-human CD152 (BNI3) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).
Flow cytometry surface staining pattern of human PHA stimulated peripheral whole blood stained using anti-human CD152 (BNI3) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).