CD142 Antibody

• Catalog Number: orb402784
• Category: Antibodies
• Description: Mouse monoclonal antibody to Tissue factor
• Target: CD142
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Human brain tissue factor (CD142)
• UniProt ID: P13726
• Tested applications: FC, ICC, IHC-P, WB
• Application notes: Flow cytometry: Recommended dilution: 3-12 µg/ml.Western blotting: Recommended dilution: 1-2 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: HTF-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: F3, tissue factor, tissue thromboplastin, coagulat
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 2152
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human PHA stimulated peripheral blood mononuclear cells using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC) and SYTO 16.

Separation of human CD142 positive lymphocytes (red-filled) from CD142 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Immunohistochemistry staining of human cortex (paraffin-embedded sections) with anti-CD142 (HTF-1), 10 μg/ml.

Western blotting analysis of human CD142 using mouse monoclonal antibody HTF-1 on lysates of A431 cell line and MCF7 and THP1 cell lines (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD142 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD142 was detected around 50 kDa.