CD142 Antibody (APC)

• Catalog Number: orb1881227
• Category: Antibodies
• Description: CD142, also known as coagulation factor III, tissue thromboplastin, and tissue factor. It is a transmembrane glycoprotein, which enables cells to initiate the blood coagulation cascades, and functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. It is the only one factor in the coagulation pathway for which a congenital deficiency has not been described.
• Target: CD142
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: APC
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: Human brain tissue factor (CD142)
• UniProt ID: P13726
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: HTF-1
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: F3, tissue factor, tissue thromboplastin, coagulat
• Note: For research use only
• Entrez: 2152
• Expiration Date: 12 months from date of receipt.

Separation of A431 cells (red-filled) from human lymfocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood spiked with A431 cells stained using anti-human CD142 (HTF-1) APC antibody (10 μl reagent / 100 μl of sample - peripheral whole blood spiked with A431 cells).

Flow cytometry surface staining pattern of human peripheral whole blood spiked with A431 cells stained using anti-human CD142 (HTF-1) APC antibody (10 μl reagent / 100 μl of sample - peripheral whole blood spiked with A431 cells).