CD141 Antibody (APC)

• Catalog Number: orb1993800
• Category: Antibodies
• Description: CD141, also known as thrombomodulin or fetomodulin, is a single chain type I transmembrane glycoprotein serving as a receptor for thrombin and as an important cofactor in the protein C anticoagulant system, but it is also involved in embryonic and atherosclerotic plaque development. CD141 is expressed mainly on macrophages, monocytes, a subpopulation of myeloid dendritic cells, on platelets and endothelial cells, but also e.g. on keratinocytes (epithelium). After binding to thrombin, CD141 activates protein C, which degrades clotting factors Va and VIIIa, and as a consequence the amount of thrombin is reduced. Mutations in the CD141 gene can cause a thromboembolic disease known as inherited thrombophilia.
• Target: CD141
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: APC
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: MV4-11 cell line
• UniProt ID: P07204
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: M80
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: Thrombomodulin, fetomodulin, THBD, THRM, THPH12, T
• Note: For research use only
• Entrez: 7056
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD141 (M80) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD141 (M80) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).