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Catalog Number | orb44692 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to CD135. |
Target | CD135 |
Clonality | Monoclonal |
Isotype | Mouse IgG1 |
Conjugation | PE |
Reactivity | Human |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Immunogen | BV-173 leukemic cell line |
UniProt ID | P36888 |
RRID | AB_10989857 |
Tested applications | FC |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
Antibody Type | Primary Antibody |
Clone Number | BV10A4 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Alternative names | Anti-CD135 antibody |
Note | For research use only |
Entrez | 2322 |
Separation of REH cells stained using anti-human CD135 (BV10A4) PE antibody (20 μl reagent per million cells in 100 μl of cell suspension, red-filled) from REH cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as CD135 PE concentration, black-dashed) in flow cytometry analysis (surface staining).
Flow cytometry surface staining pattern of REH cellular suspension stained using anti-human CD135 (BV10A4) PE antibody (20 μl reagent per million cells in 100 μl of cell suspension).