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Catalog Number | orb421967 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against CD134 conjugated to FITC |
Clonality | Monoclonal |
Clone Number | Ber-ACT35 |
Tested applications | FC |
Reactivity | Human, Primate |
Isotype | Mouse IgG1 kappa |
Immunogen | HTLV 1-transformed HUT-102 cells |
Antibody Type | Primary Antibody |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | CD134 |
Entrez | 7293 |
UniProt ID | P43489 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-TNFRSF4 antibody, anti-OX40 antibody, anti-AC Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD134 (Ber-ACT35) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD25 (MEM-181) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD134 positive CD25 positive cells (red-filled) from CD134 negative CD25 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD134 (Ber-ACT35) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).
Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD134 (Ber-ACT35) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).