CD133 Antibody (APC)

SKU: orb545655

Description

Mouse monoclonal antibody against CD133 conjugated to APC

Research Area

Epigenetics

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: W6B3C1
• Immunogen: WERI-RB-1 retinoblastoma cell line
• Target: CD133
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Prominin 1, PROM1, AC133, PROML1, STGD4

Flow cytometry multicolor surface staining of human bone marrow cells stained using anti-human CD133 (W6B3C1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD34 (581) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).

Separation of human CD133 positive CD34 positive stem cells (red-filled) from CD133 negative CD34 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human bone marrow cells stained using anti-human CD133 (W6B3C1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Flow cytometry surface staining pattern of human bone marrow cells stained using anti-human CD133 CD133 (W6B3C1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Quick Database Links

Gene Symbol

CD133

UniProt

O43490

NCBI Gene ID

8842