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Anti-Hu CD122 FITC
Catalog Number: orb2704158
| Catalog Number | orb2704158 |
|---|---|
| Category | Antibodies |
| Description | CD122 (IL-2/IL-15R beta) constitutes together with CD132 (common gamma chain) and with CD25 (IL-2/IL15R alpha) the intermediate (CD122+CD132) and the high affinity (CD122+CD132+CD25) IL-2 and IL-15 receptor complex. CD122 is expressed on NK cells and lymphocytes, but at low level, unless the cell is activated. The cytoplasmic part of CD122 binds to Src-family and Jak-family kinases. The biological effect of CD122 ligation depends on whether IL-2 or IL-15 is bound to the receptor complex. |
| Target | CD122 |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Conjugation | FITC |
| Reactivity | Human |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purification | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Immunogen | TL-Mor cell line |
| UniProt ID | P14784 |
| Tested applications | FC |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Clone Number | TU27 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative names | IL2RB, IL15RB, P70-75 |
| Note | For research use only |
| Entrez | 3560 |
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD122 (TU27) FITC antibody (concentration in sample 0.56 µg/ml).
Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD56 (LT56) APC antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human CD122 (TU27) FITC antibody (concentration in sample 0.56 µg/ml).
Separation of human CD122 positive CD56 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD122 (TU27) FITC antibody (concentration in sample 0.56 µg/ml).
