CD122 Antibody (Biotin)

• Catalog Number: orb2704161
• Category: Antibodies
• Description: CD122 (IL-2/IL-15R beta) constitutes together with CD132 (common gamma chain) and with CD25 (IL-2/IL15R alpha) the intermediate (CD122+CD132) and the high affinity (CD122+CD132+CD25) IL-2 and IL-15 receptor complex. CD122 is expressed on NK cells and lymphocytes, but at low level, unless the cell is activated. The cytoplasmic part of CD122 binds to Src-family and Jak-family kinases. The biological effect of CD122 ligation depends on whether IL-2 or IL-15 is bound to the receptor complex.
• Target: CD122
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: Biotin
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Immunogen: TL-Mor cell line
• UniProt ID: P14784
• Tested applications: FC, IP
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Clone Number: TU27
• Storage: Store at 2-8°C. Do not freeze.
• Alternative names: IL2RB, IL15RB, P70-75
• Note: For research use only
• Entrez: 3560
• Expiration Date: 12 months from date of receipt.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD122 (TU27) biotin antibody (concentration in sample 0.56 µg/ml, Streptavidin APC).

Separation of human CD56 positive NK cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD122 (TU27) biotin antibody (concentration in sample 0.56 µg/ml, Streptavidin APC).

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD56 (LT56) PE antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human CD122 (TU27) biotin antibody (concentration in sample 0.56 µg/ml, Streptavidin APC).