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Catalog Number | orb44137 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against CD11b conjugated to Biotin |
Clonality | Monoclonal |
Clone Number | MEM-174 |
Tested applications | FC, IP |
Reactivity | Human |
Isotype | Mouse IgG2a |
Immunogen | Human granulocytes |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Purity | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
Conjugation | Biotin |
Target | CD11b |
Entrez | 3684 |
UniProt ID | P11215 |
RRID | AB_10990666 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | anti Antigen CD11b p170 antibody (Biotin), anti CD Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: peripheral blood lymphocytes. |
Expiration Date | 12 months from date of receipt. |
Separation of human monocytes (red-filled) from CD11b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11b (MEM-174) Biotin antibody (concentration in sample 6 µg/ml, Streptavidin APC).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD11b (MEM-174) Biotin antibody (concentration in sample 6 µg/ml, Streptavidin APC).